effects of different doses of bone morphogenetic protein 4 on viability and proliferation rates of mouse embryonic stem cells

objective: in this study, we examined the effect of different doses of bone morphogenetic protein 4 (bmp4) on cce mouse embryonic stem cells (escs) viability and proliferation rates in order to improve the outcome of induction processes and make a system with highest viability and proliferation rates for further studies on bmp4 roles in multiple developmental stages. materials and methods: expression of oct-4 was studied and confirmed in this cell line immunocytochemically. also, in order to evaluate the proliferation and viability rates in bmp4-treated cells, escs were cultured in dulbecco's modified eagle medium (dmem) containing different doses of bmp4 (0, 0.01, 0.1, 1, 5, 25, 50 and 100ng/ml). the mean number of whole cells and living cells were considered as proliferation and survival rates respectively. data analysis was done with anova test. results: the results showed that there were significant differences between the mean percent of viability between 1ng/ml and 0 ng/ml (control) and 50 and 100 ng/ml bmp4 (p≤0.01), as well as between 5 ng/ml and 0, 0.01, 0.1, 25, 50 and 100 ng/ml bmp4 (p≤ 0.02). also, significant differences were observed in proliferation rates between 5 ng/ml and 0, 0.01, 0.1, 1, 25 and 100 ng/ml bmp4 (p≤0.01), 25 ng/ml and 0.01, 1 and 5 ng/ml bmp4 (p≤0.01), as well as between 50 ng/ml and 0.01 and 0.1 ng/ml bmp4 (p≤0.001). conclusion: the results suggest that addition of 5ng/ml bmp4 had the best effects on the proliferation and viability rates of cce mouse escs.